Department of Microbiology
We will be exploring the utility of small bio-reactive molecules for use in controlling viruses and protozoan pathogens without harming bees.
The world fisheries production has levelled off and most of the main fishing areas have reached their maximum potential. In contrast, the global human population is increasing; thus, the demand for aquatic food products also increase. Global aquaculture production attained 90.4 million tons in 2012, generating an incomes US$ 144.4 billion, and the production of food fish was 66.6 million tons. Epitheliocystis is a serious skin and gill disease in fish, believed to be caused by pathogenic intracellular bacteria.
The diverticulated crop organ of the common house fly, which is the major insect vector of numerous human food pathogens (e.g., Escherichia coli) is the major reservoir or storage area for this, and other, important food pathogens. It has also been demonstrated that this is where horizontal transmission of antibiotic resistance to E. coli occurs. Thus, the diverticulated crop organ is an essential component in the transmission cycle between pathogens and human foods/food crops.
Our goal is to evaluate the role and causative mechanisms of parasitic mites, viruses, and microbes in pollinator abundance and honeybee colony success. Isolation of total RNA and DNA from bee guts will be performed following standard methods currently used in our laboratory. Bee infection status with viruses and the eukaryotic parasites Crithidia and Nosema will be determined by PCR and rtPCR analyses to detect viruses and parasites using RNA and/or DNA extracted from guts as template.
Only 10 years ago the "brown rot" wood degrading fungi were considered to be poorly evolved organisms in the fungal world. It was known that they lacked many of the enzymatic systems that the taxonomically more numerous "white rot" wood degrading fungi possessed, and it was thought that the brown rot fungi just had not yet reached a stage of evolution to produce these enzymatic systems. In essence, they were thought to be more primitive fungal organisms. However, new genomic analyses conducted over the last 7-8 years have turned this thinking on its head.
Nearly all food and agricultural waste in the U.S. enters landfills, making it the largest contributor of material entering these sites. Biological pre-treatment of large organic molecules by fermentative organisms lowers the high organic carbon load in waste, lowers wastewater treatment costs, and can produce bioenergy to partially offset costs. Conceivably, microbes that grow best above 80°C, or so-called ‘hyperthermophiles’, could be used to consolidate wastewater heat treatment and organic remediation in a single step to decrease costs while producing H2 as an energy product.