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Massachusetts Agricultural Experiment Station

Timothy and alfalfa are important forage crops that are fed to domestic animals. In future climate change, they will be exposed to elevated concentrations of carbon dioxide (CO2) and ozone (O3). We know little about how common varieties of these perennial plants respond to current levels of CO2 and O3. We do not know how cultivars of these plants would respond to future elevated levels of CO2 and O3.Plants will be exposed to current and future levels of CO2 and O3 in greenhouse exposure chambers.

In an effort to reduce dependence on petroleum, promote economic growth and diversification, and reduce human-induced climate change, the United States has developed a strategy that includes bio-based production of energy and compounds that can be used as precursors for industrial processes. It has been suggested that microorganisms with differing physiological capacities may provide an opportunity to generate commercially valuable products in a more sustainable, commercially viable manner.

Tree growth, post-establishment [defined as a resumption of pre-transplant growth rate (Struve and Joly 1992)], will be determined by measuring caliper of the established 48 research oak (Q. bicolor, Q. rubra) trees, annually for the next four years (preferably longer, but contingent upon funding). Annual increment will be analyzed using piecewise regression.

The market for specialty foods targeted for Latino populations is especially strong in New England. While most of these foods are imported, many can be locally grown, offering a strong and increasing market for local farmers. We will work to evaluate germplasm of Latin American specialty crops with the objective of identifying parental materials to initiate a breeding program for these crops.

In vitro maturation, in vitro fertilization, and embryo development will be according to procedures widely published in the literature and frequently used in the laboratory. In vitro maturation and fertilization will be conducted in 400 μl drops at 38.5°C and 5% CO2 in a humidified atmosphere, while embryo culture will be carried out in 100 μl drops under mineral oil of KSOM– 0.4% BSA medium (EmbryoMax, Chemicom International, USA) at 38.5°C and 5% CO2, 5% O2 and 90% N2, in a humidified atmosphere.

The world fisheries production has levelled off and most of the main fishing areas have reached their maximum potential. In contrast, the global human population is increasing; thus, the demand for aquatic food products also increase. Global aquaculture production attained 90.4 million tons in 2012, generating an incomes US$ 144.4 billion, and the production of food fish was 66.6 million tons. Epitheliocystis is a serious skin and gill disease in fish, believed to be caused by pathogenic intracellular bacteria.

Family forest owners (FFOs) control 263 million acres (or 35%) of U.S. forests. In the eastern U.S, FFOs control more than 50% of the forests (Butler, 2008). The average age of FFOs is over 60 years old. It is estimated that over 75% of family forest land is owned by people over the age of 55 and nearly 50% is owned by people over the age of 65 (Butler, et al. 2016). In the coming years, nearly 3.8 million FFOs will be deciding the future of their land. We are, in fact, in the midst of the largest intergenerational shift of land our country has ever experienced.

A male factor is responsible for about half of the cases of equine infertility: this fact highlights the need for a better understanding of the molecular basis of male equine infertility both at the genetic as well as at the biochemical level.This project is relevant for the study of male infertility from several perspectives: 1) results of the experiments will provide a significant advance in understanding at the molecular level the mechanisms of the causes of male infertility.

There is concern over the presence of pollutants in our water supply. Among the pollutants that have elicited the greatest alarm are those that can alter the development and reproduction of aquatic organisms and potentially harm human health. These chemicals, generally referred to as endocrine disrupting compounds (EDCs), enter waterways as runoff from farms, effluent from wastewater treatment and manufacturing plants, and leachate from septic systems.

This project will develop and diversify Crambe (an oilseed crop) and brassica (mustard green) species as dedicated bioenergy crops for biodiesel production. The proposed strategy will increase crop biomass and seed yields while growing these crops on marginal and heavy-metal-contaminated lands, thus increasing both yield and arable acreage. This approach to cultivation on contaminated sites and marginal lands will not create competition for land or displacement of food crops.

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