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What are Biofungicides?

Biofungicdes are formulations of living organisms that are used to control the activity of plant pathogenic fungi and bacteria. The concept of biofungicides is based upon observations of natural processes where beneficial microorganisms, usually isolated from soil, hinder the activity of plant pathogens. Biocontrol microorganisms are free-living fungi, bacteria, or actinomycetes that are active in root, soil, and foliar environments.  These microorganisms produce a wide range of antibiotic substances, parasitize other fungi, compete with other fungi, and induce localized or systemic resistance in plants. The use of composts and suppressive growing medium, which both contain living microorganisms, to mitigate disease is another example of this disease management option.

How Biofungicides Work

  • Rhizosphere Competence - The most successful of the strains of biocontrol microorganisms exhibit rhizosphere competence, the ability to colonize and grow in association with plant roots. They can colonize entire root surfaces for several months.  Here they effectively compete with plant pathogens for nutrients, infection sites, and space. Competition for glucose in the soil is involved in disease suppression.  Biofungicide organisms also metabolize seed and root exudates that normally stimulate pathogen germination or zoospore attraction.
  • Parastitism - Parasitism, the ability of species to attack and consume plant pathogens, has been well studied. Mycoparasitism of biocontrol microorganisms includes directed growth, contact and binding, coiling of hyphae around the host fungus, penetration, and degradation. Production of cell wall degrading enzymes is almost always part of the process.
  • Antibiosis - Antibiosis occurs when one microorganism produces molecules that directly affect other organisms negatively by toxicity or growth inhibition. These compounds are called antibiotics and are commonly produced by a wide range of soil dwelling microorganisms in the course of their growth. A familiar antibiotic, streptomycin, is produced by Streptomyces species which are classified as actinomycetes or filamentous bacteria.
  • Inducing Metabolic Changes - An important mechanism of biocontrol microorganisms is the ability to induce metabolic changes in plants that increase their resistance to a wide range of plant pathogenic fungi and bacteria. Systemic Acquired Resistance (SAR) improves the plant response to pathogen attack by priming the production of plant defense compounds. This capacity to induce resistance to a wide range of diseases in a variety of plants appears to be widespread. Enhanced resistance is systemic because disease resistance occurs at sites distance from the location of the biocontrol microorganisms.
  • Plant Growth Promotion - A final way in which these organisms act is through plant growth promotion. Beneficial root-colonizing microorganisms promote plant growth and productivity. Many resistance-inducing fungi and bacteria promote both root and shoot growth in the absence of plant pathogens. When applied to growing media or as a seed treatment, some biofungicides can increase root development and improve drought resistance in some plants.  Improvements in plant growth result from effects on soil microflora and direct effects on the plant. Biofungicides can also improve nutrient uptake (copper, phosphorous, iron, and manganese).

Biofungicides Used in Greenhouse Production

Plant Shield, Root Shield, PreStop, SoilGard, Asperello T34, and T-22 PlanterBox are commercial formulations of Trichoderma species and the closely related Gliocladium species. Species of Trichoderma are the best studied of all biocontrol organisms. These species are also abundant in many types of compost and are the most frequently isolated soil fungi from all temperate and tropical soils. The production of antibiotics by these species is well documented.  In fact, Trichoderma species have been found to be highly resistant to a variety of toxins and antibiotics produced by soil microorganisms, antimicrobial compounds produced by plants, and even chemical fungicides. This makes these fungi active colonizers of toxic environments and a strong competitor.

Companion, Cease, and Sonata contain bacteria in the species Bacillus. Many biofungicides that based on bacteria are labeled mainly for soilborne diseases, though there are some that are also labeled for foliar diseases like leaf spots, powdery mildew, and downy mildew (see table below). Other bacteria are termed Plant Growth Promoting Rhizobacteria (PGPR) and while they do not have any direct effect on pathogens their growth promoting effects can contribute to disease tolerance. Actinovate, Actino-Iron, and MycoStop are composed of species of Streptomyces, an actinomycetes or filamentous bacteria. They are labeled for both root and foliar diseases.  Agrobacterium radiobacter 84 (Galltrol A) is registered for control of Crown Gall (Agrobacterium tumefaciens) and there are also a few Pseudomonas species present in some biofungicides (Blight Ban and Bio-Save 10 LP).

How to Use Biofungicides

In the greenhouse industry, biofungicides are applied preventively to growth media or as a seed treatment for root and crown disease control and can be as effective as chemical fungicides. Some growers have had good success with dipping plugs in biofungicide solutions prior to planting. Biofungicides used for foliar disease management must be applied preventatively. Biofungicides are generally safer for growers, can be more persistent, and are sometimes less expensive than conventional fungicides.

The most effective use of biofungicides is as a preventive treatment in growing media or as a seed treatment. They should be mixed into the growing media prior to planting or applied as a drench immediately after transplanting, making sure that the entire soil volume is treated. For foliar applications, the biofungicides must be in place before pathogen infection as their action is purely protective. They must be reapplied frequently both to protect new growth and to ensure that effective populations of the microorganisms are present. Because biofungicides consist of living organisms, they may have different storage, shelf life, and handling requirements than conventional fungicides. Most biofungicides have short reentry intervals (0-4 hours). Always read the product label and follow instructions carefully. Biofungicide manufacturers are typically the best source of information on how to optimize the use of their products.

It is always advisable to wear protective clothing and eyewear when handling pesticides, and this includes biofungicides. Consider using a dust mask when handling powdered formulations.

Biofungicide Products Labeled for Greenhouse Crops. All products are labeled for most greenhouse ornamentals and vegetables. See product labels for more specific information. Always follow label instructions.





Streptomyces lydicus

Powdery mildew, Downy mildew, Botrytis, Rhizoctonia, Pythium, Phytophthora


Ulocladium oudemansii U3

Botrytis, Sclerotinia

Cease, Rhapsody

Bacillus subtillis QST 713

Rhizoctonia, Pythium, Phytophthora, others

Companion Liquid

Bacillus subtillis GB03

Leaf spots, Powdery mildew, Botrytis, bacterial diseases, Rhizocotonia, Pythium, Phytophthora

Contans WG


Coniothryium minitans

Sclerotinia sclerotiorum, S. minor

Double Nickel, Triathlon

Bacillus amyloliquefaciens

Powdery mildew, Downy mildew, Botrytis, Rhizoctonia, Pythium, others


Agrobacterium radiobacter K84

Agrobacterium tumefaciens


Streptomyces griseoviridis

Botrytis, Rhizoctonia, Pythium, Phytophthora, Alternaria

Plant Shield,

Root Shield,
T-22 Planter Box

Trichoderma harzianum

Rhizoctonia, Pythium, Fusarium, Cylindrocladium, Thielaviopis

Prestop WP

Gliocladium catenulatum JII446

Botrytis, Rhizoctonia, Pythium, Phytophthora, Fusarium, Verticillium


Gliocladium virens GL-21

Rhizoctonia, Pythium

by M.Bess Dicklow, 9/2014. Updated by Angela Madeiras, 7/2018.