This research involves utilizing genomics and molecular biology tools to understand the basis of DMI (demethylation inhibitor) fungicide resistance dollar spot, the most important disease of turf grasses for golf courses. For this, we will take advantage of cutting-edge research tools in genomics and molecular biology to shed light on how the dollar spot fungus is able to overcome fungicides at the molecular level. Benefits from this work include new targets for fungicide development, new targets for diagnostic assays to detect fungicide resistance, and a better scientific understanding of the molecular basis of DMI fungicide resistance.