Oil seed and other food crops will be grown under various environmental stress conditions, RNA/DNA will be extracted and differential regulation of genes will be studied to understand the mechanisms at molecular levels. Identified candidate genes will be characterized by overexpression and/or knockdown in crops using genome-editing approaches via plant tissue culture methods. The resulting edited crops will be analyzed for improved agronomic traits and stress tolerance under greenhouse conditions using molecular and biochemical assays. These are standard and routine methods used in Dr. Dhankher’s laboratory. The results will be published in high impact journals and disseminated at various regional, national and International conferences. Students will be trained on this project.